Applied Microscopy and Cell Biology

The laboratory offers the development of new protocols and new cell models for biocompatibility, toxicity, regenerative medicine studies using the latest and advanced instruments.

Activities

Cellular Models to evaluate Compounds Biocompatibility and Toxicity :

    • Evaluation of biomedical devices biological safety (ISO10993 guidelines)
    • Biocompatibility: In vitro tests for the evaluation of cytotoxicity, according to ISO10993-5 standard and/or by using customized alternative approaches.
    • In vitro tests for genotoxicity and mutagenesis assessment (in vitro mammalian cell gene mutation tests using the thymidine kinase gene; OECD guidelines for testing chemicals)
    • Cell culture: the MAB laboratory is specialized in human mesenchymal stem cells isolated from adipose tissue or bone marrow culture. It is also expert in cell cultures preparation  (primary and stabilized, human and not human ) isolated from healthy or pathological tissues, and in pluripotent cells engineering  (iPS).
    • LABEL FREE ANALYSIS: we are able to use this innovative technology that allows to monitor in real time the biological behavior of cells following interaction with drugs, molecules, eluates and substances of interest. The advantage of the assay consists in the possibility of carrying out a rapid predictive screening using cells from the tissue of interest.
    • CELLULAR-MATERIAL INTERACTION: we set up three-dimensional cell cultures on biomaterials and biomedical devices to evaluate cytotoxicity and biocompatibility. Thanks to advanced systems of optical microscopy (Zeiss) it is possible to understand how the cell and the biomaterial influence each other.
    • BIOPRINTING: In collaboration with the MS2 laboratory, it is possible to print both cells and materials to obtain innovative cellular and tissue models able to mimic the physiology of organs and tissues. The ultimate goal is to predict safety, biocompatibility and effectiveness of new therapeutic approaches.

Application sectors

Dental
Pharmaceutical
Cosmetic
Agro-food
Biomedical

Regenerative Medicine:

Identification of the cell-biomaterial combination, which allows the differentiation towards a target tissue.

MAB has experience in:

  • pre-clinical studies for the regeneration of bone and adipose tissue
  • Writing and submission of studies to the ethics committee for in vivo experiments
  • In vivo damage simulation of human pathologies and treatments  with stem cells and biomaterials in autologous, allogeneic and xenogenic settings
  • assessment of the implant through histological investigations

Application sectors

Pharmaceutical
Veterinary

Microscopy, Image Analysis, Histology:

The advanced microscopy branch of the MAB laboratory is able to answer to different company needs through :

  • histopathological investigations for the physio-pathological characterization of tissues
  • surface studies of materials by large surfaces scanning with high resolving power and  reconstruction of the image in three dimensions
  • biomaterials colonization with fluorescent cells
  • image analysis to transform qualitative data into a semi-quantitative survey

We deliver tailor made approaches through:

  • creation of three-dimensional models of bacterial biofilms on biomedical devices for the evaluation of sterilization processes or new functionalizations of surfaces,  in vivo and in vitro.
  • biochemical tests for the study of inflammatory processes in various stages of treatment.
  • blood simulation models to detect possible blood parameters changes in and activation of coagulation processes.
  • functionalization and characterization of membrane performances for biomedical devices.
  • support  in the R & D phases.

Application sectors

Cosmetic
Agro-Food
Veterinary

Analysis of bacterial biofilms on biomedical devices surfaces :

It is possible to evaluate the bacterial biofilms formation on a biomedical device through in vitro and in vivo investigations, monitoring the same animal with in vivo imaging. Microscopy techniques can also be applied to study the biofilm distribution in the device.

Application sectors

Biomedical
Ceramic-Building

Scientific director

Prof. Massimo Dominici MD

Laboratory manager

Dott. Elena Veronesi PhD


Staff and skills

Dott. Elena Veronesi: Laurea in Scienze Biologiche, PhD in Medicina Molecolare e Rigenerativa
Dott. Elisa Resca: Laurea in Biotecnologie Mediche, PhD in Scienze Morfologiche Umane e Molecolari
Dott. Tiziana Petrachi: Laurea in Biotecnologie Mediche e Farmaceutiche, PhD in Medicina Molecolare e Rigenerativa
Dott. Maria Serena Piccinno: Laurea in Biotecnologie Mediche, esperta di colture di cellule staminali e label free analysis
Dott. Valentina Strusi: Laurea in Biotecnologie Mediche, Veterinarie e Farmaceutiche, PhD in Scienze Biomediche

Instrumentation

Axiozoom V16 (Zeiss) optical microscope that combines both stereomicroscope and confocal features. Versatile tool, suitable for many applications, for surface observation of plastics, organic materials, metals, cements, biological material adhered to materials, cell cultures.

IVIS LUMINA III (PerkinElmer) for in vivo imaging on mice, equipped with fluorescence, X-ray luminescence.

Enspire multi-plate reader (PerkinElmer) for evaluation of cytokines released following stimulation, evaluation of dye concentrations, label free assays, receptor activation studies.

Instrumentation for cell cultures: cell cultures hoods, incubators, centrifuge, bath thermostat, tissue digestion shaker, dedicated refrigerators, inverted optical microscope (Observer, Zeiss) and EVOS (life technologies), freezer -80 and nitrogen plant for cell preservation.

Station for paraffin embedding: automatic inclusor, microtome, fluorescence-equipped optical microscope (Imager, Zeiss)