MAB-MICROSCOPY AND BIOLOGY

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MAB
APPLIED MICROSCOPY AND CELLULAR BIOLOGY

Scientific Director: Prof. Massimo Dominici

TECHNICAL SCIENTIFIC SERVICES:

> Cell cultures in two-dimensional supports;
> 3D matrix cell cultures;
> Isolation, expansion and differentiation of human stem cells ;
> Isolation, expansion and differentiation of induced pluripotent stem cells (iPSC);
> Optical microscopy applied to materials and biological samples;
> Morphological analysis of material;
> Molecolar Biology and quantification of the biologic solutes released during cell-biomaterial contact;
> Nucleic acids amplification, analysis of mRNA expression, ELISA test, AlphaLisa e label free;
> Flow cytometry and cell sorting analysis;
> Pre-clinic studies trough in vivo imaging;
> Consulting upon biocompatible matrices optimisation for a regenerative medicine approach.

 

APPLIED RESEARCH:

> Use of stem cells as innovative biocompatibility sensors, as well as specific tissues;
> Support for biomed devices development for regenerative medicine;
> Support for point-of-care biomed devices development;
> Biocompatibility assessment between biomaterials and biological matrices;
> Assessment of medical devices efficacy using methods based on direct contact between cells and biological tissues in vitro and in vivo;
> Introduction of in vitro predictive toxicity tests for pharmaceutical ingredients in development;
> Development and safety tests for new product and processes, data analysis, reports and papers editing.

EQUIPMENT

Cell Cultures
> Ice machine
> CO2 incubator
> Cryoplus_ Device for cryoconservation in liquid nitrogen
> Freezer -80°C
> Class II biohazard cabinet

Histology 
> Fume cupboard
> Paraffin infiltrator
> Paraffin bath
> Dispensing console
> Microtome

Molecular Biology and Biochemistry
> Transilluminator
> Laminar flow cab PCR
> Thermal cycler
> Electrophoresis set and Image acquisition system
> Microplates reader

Applied Microscopy
> Microscope AXIO Imager M2
> Microscope AXIO Zoom V16
> Microscope AXIO Observer Z1

CONTACTS

Cristina Simonato 

Mail: cristina.simonato@tpm.bio

Phone: +39 0535 613810

Marco Turci

Mail: marco.turci@tpm.bio

Phone: +39 0535 613811